Lab Operations and QC.

What stuffs I need to know from Lab Operations and QC?
Accuracy

Precision

Standard Deviation

Sensitivity

Specificity

Controls= Assayed and Unassayed

Delta Check

Youden Plot for?

Levey-Jennings= know Westgard Multi Rules

Know some common Maths like Molarity, Dilution,

Know about Lab Safety like Fire safety, Radiation sign, Bio Hazard sign, Fire extinguisher classes, Biological safety Cabinets, Accrediated Agencies.

KNow just the principles of Instrumentation, All you need to know the basic idea about instrument, just 2-3 lines—> Spectrotophotometry(stary light and filetr?)= for example this method measures light in a Narrow Wavelength Range.

Fluorometry

Turbidimetry

osmometry

chromatography

Electrophoresisi

Potientiometry

coulometry

Amperometry

1. Accuracy *Percentage of correct diagnoses
*Total Correct Scans/Total # of Studies
*Accuracy= TP=TN/ALL Scans
(note ALL Scans are TP+FP+FN+TN)
A+D/A+B+C+D
2. False Negative (for example: cutting off the wrong leg)
Scan disagree with gold standard
-Scan normal
-Gold standard abnormal
3. False Positives (for example: taking medicine you don’t need)
Scans disagree with gold standard
-Scan abnormal
-Gold standard normal
4. Negative Predictive Value *Percentage of scans that accurately predict normality
*TN/ALL
(note ALL negatives are TN +FN)
NPV=TN/(TN+FN)
D/C+D
5. Positive Predictive Values *Percentage of scans that accurately predict abnormality
*TP/ALL
(note ALL positives are TP + FP)
PPV=TP/TP+FP
A/A+B
6. QA Statistics * Compare your exam to the gold standard or reference test
* Calculated values verify
-Quality of your exams
-Limitations of your lab
7. Sensitivity TP/(TP +FN)
*Identify patients with disease
Sensitivity = A/A+C
8. Specificity TN/(TN+FP)
*Identify patients without disease
Specificity = D/B+D
9. Test Validation and Statistics * Sensitivity
* Specificity
*Positive Predictive Value
*Negative Predictive Value
*Accuracy
10. True Negative (Normal or No Pathology)
Your scans and the gold standard agree
11. True Positives (Finding the presence of disease)
Your scans and the gold standard agree

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