Some joke time, A Doctor calls the Lab and needs Stat Blood Culture grown in 30 mins.
Boc Micro–> boc5 microbiology
Microbiology and Immunology Text Book —> MICROBIOLOGY & IMMUNOLOGY-1
Table # 1 Gram Positive Catalase (+) Bacteria
From Table #1 I got 3 Questions, those which are in red Squared.
Lets Summarise Table #1 and #2
1) Bile Esculin Agar= Group D Strep and Enterococci are positive
2) 6.5% Nacl Broth= Only Enterococci grow, Group D Strep Does not.
3) Optochin Disk = Strep. Pneumoniae is sensitive, Strep Viridian is Resistant. Remember (oPtochin has letter P and pneumonia also starts with P )
4)Bile Solubility: S. pneumonia is Bile soluble.
5) Bacitcin disk sensi= Group A Strep.
6) SXT = Both Group A and Group B strep are Resistant.
7)Novobiocin= Staph. Epi is (+) whereas Staph Saphro is (-)
8) PYR + = Group A strep and Enterococci
9) Campt Test= Strep Group B positive.
10) Hippurate Hydrolysis= Greoup B Strep (+)
Table # 3 Gram (+) Bacillus
Lets Summarize Table # 3
Listeria= Cold Enrichment, Tumbling Motility, Umbrella Growth. Motile at 25 degree Centigrade but not at 37 degre. The only Motile one among Gram pos Bacillus Aerobic Bacteria.
Nocardia= Branched, Partially Acid Fast like Mycobacterium.
B. Anthracis= Spore Forming, Ground Glass Bambmoo shoot like structure, Medussa Head, Causes Anthrax and cutaneous ulcer from handling wool hides in Live Stock.
B Cereus = So this Organism is responsible for Food Poisoning, Toxin Fried Rice.(Just Remember Morning Cereal as food and name of this organism is also Cereus similar name and it causes Food poisoning)
Lactobacillus= Pleomorphic, Flora of Vagina.
Erysipelothrix= H2S+, So causes skin infection in Fisherman or Butchers or rose Growers, In short it causes Occupational infection.
Corynebacterium= Chinese letter like, Black Colonies on Tindale Agar or Loeffler Agar Medium. , Confirm by growth in Potassium Tellurite Agar . Highly Toxic Organism. Need to confirm toxicity by ELEK test to check if organism is a producer of toxin, if it produces then you can report that Species is Toxic.
Table # 4 Gram Negative Enterobactericae
Summary of Table # 4
1) Lactose Ferm= E Coli , Enterobacter,Klebsiella = Mucoid, Citrobacter= slow Fermenter.
Klebiseilla has 2 types and can be differentiated on the basis of Indole Test. Kleb Oxytoca is Indole + and Kleb Pneumina is Indole -, How will you remeber which one is Ibdole (+)—-> just remember Oxytoca like oxygen and so indole + 🙂
2) Non Lactose Fermenter= Salmonella, Shigella, Proteus, Morgan and Pseudomonas.
Remember Proteus has 2 types and can be differentiated on the basis of Indole Test
Proteus Vulgaris is Indole + and Port Mirabilis is Indole –
So how do I remember which is positive , just compare Vulgaris with Volcano and its hot and firy and so Indole +
3) TSI reactions = A= Acid means Ferment lactose, sucrose or Glucose
Alkaline =K= No Fermentation.
A on Top means ferment Lactose or Sucrose, A on Bottom means Ferment Glucose. A/A = Ferment through out Slant (Body) and Butt (Bottom)
K/A= means does not ferment Lactose but just Ferment Glucose.
SO look at the Table #4 and see the TSI table.
Colum 1 =A/A = EEKY = first 3—> EEK are Lactose Fermenter and Yersenia just Ferment Sucrose, so the Agar plate with EEK will be pinkish but not with Yersenia bcos its not Lactose Fermenter.
2nd Column = A/A and H2s+ = Citrobacter.
3rd column = K/A= Non Lactose Fermenter , just Glucose Fermenter and does not produce H2S (negative)= SS-PP-M
4th Column= K/A and H2S+= Saci-Pred= Salmonella, Proteus and Mr. Edward 🙂
I just kept Citrobacter so you can remeber that Saci-Pred= H2S+ but Citrobacter had already been placed in column 2 bcos its slow lactose fermenter and have TSI of A/A type.
Column 5= K/K= zero nothing, No fermentation and No H2S=Pseudomonas. Only Organism which is Oxidase + among this group.
4)Phenyl De Aminase +=PD+=PPM=prot, prov and Morganella.
5)Urease += same like PPM just add K= Klebsiella so= PPM-K = Prot, Prov , Morgan and kleb.
Special case: But remember not all of providencia Species are Urease (+) only and only Providencia Rettgeri among the Providencia Species is urease (+) and also Klebseilla is Slow Urease +
6)Citrate += Enter-SePr/K = entero, Serratia, Proteus and Klebsiella.
7)Oxidase + = The ones which end with (-onas)= Pseudomonas and Pleisimonas.
8)H2S+= Saci-PrEd= sexy predator= Sal, Citro, Proteus and Mr. Edwardsiella.
9)Non Motile = The ones ending with (-ella)= Klebsiella and Shigella :)_
Remember how Klebsieilla is pronounced Kleb-siiieeellla takes a long time to read so this organism is very mucoid and cannot move bcos it takes long to read 🙂
10)Indole += E coli , K Oxytoca (remember as Oxygen), P. Vulgaris (Volcano), Mr. Edward and Mrs Morgan (they both work at my Lab 🙂 ) and finally 2 of which start with P i.e—> Providenscia and Pleisimonas. 🙂
Summary of Table #6:
These Bacteria will always Grow on Choclate AGar and 10 % Co2 because it supplies both X and V Factors. They do not Grow on Blood Agar because of NADase in the agar ( which inactivates NAD) but will grow on Horse or Rabbit Agar which contains no NADase.
Exception on Growing in Blood Agar can happen only when this bacteria grow around colonies of S Aureus as S. Aureus produces NAD and Hemolyse the Blood cells releasing Hemin as well and this Phenomenon is known as Satellitism.
Both H Influenzae and H. Haemolytics (Not Para’s) need both X and V Factor
Both Haemolytics (Haemolytics and ParaHaemolytica)= Hemolyse
Both Para (Para Influenz. and Para Haemolytia) need V (NAD) and don’t need (X)
H.Aphrophilus and H Ducreyi = Reactions are exactly opposite For H Aphro= – – – + and H Ducreyi = + – – –
Diplococci = Neisseria Species and Moraxella
Neisseria Species Grow on Choclate and selective Media ( MTM, Martin Lewis and NYC)
Neisseria Gonnorhoea Very Resistant ot Penicillin and causes STD
All 3 Neiserria’s Ferment GLucose
Gonnorhea = Only Glucose
Meningitis= starts with M SO ferments Glucose and Maltose
Lactamica = Starts with L and has M in the middle= SO Fermenrs Glucose + Maltose+ Lactose
Summary: Fusobacterium = Fus Fus= Brain Abcess, spindle shaped (brain like structure so attack Brain 🙂 )
Last 3 causes Pulmonary Infection and Bacteroides infects below Diaphragm
Brick Red Flouresce = Porphyromas, Prevotella and Veillonella
Indole (+)= Fuso Bacterium and Porphyromas and Both organsims are sensitive to 2 Antibiotics
Extra Notes: Miscellaneous
MicroBiology Review in a Nutshell :